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Stable Isotope help

Daniel Danckwerts, June 6, 2013
 

Hi all, I am working to describe the diet of Barau's Petrel at Reunion Island.To do this i will use stable isotopes, fatty acids and stomach contents.

My question is this: i have spun blood (plasma and RBC) samples to try and understand the dual foraging behavior of this species. For whole blood we use between 1 and 1.2mg of sample for stable isotope analyses. I have not been able to find anything on the weights used for plasma and RBC. How much plasma an red blood cell do you weight out for the stable isotope analysis?

Comments ( 4 )

Grant RW Humphries

Grant RW Humphries

Hey Daniel, I would contact Alex Bond for a start: alex.bond@usask.ca

He would be a good place to start, and I'm sure he can point you in the right direction! :) Good luck

Marcelo Bertellotti

Marcelo Bertellotti

I would contact Manuela Gonzalez Forero (gforero@ebd.csic.es) who has extensive experience in the field. Good Luck!

Viviane Barquete

Hi Daniel,

I worked with the SIA on my PhD thesis and I used to weight between 0.6-0.7 mg for blood tissues (total blood, red cells, plasma) and feathers. So, if you are weighting around 1-1.2 mg for total blood, I believe you can use the same amount for plasma and red cells.

Hope it helps and good luck!

Alex Bond

Alex Bond

It all depends on your lab's setup. Some places need as little as 0.2 mg, others need 1.0 mg. The mass required depends on the abundance of bulk C and N (plants have low N, for example, so you need to weigh out more than animal protein). My guess is that if your lab needs 1.0-1.2 mg of whole blood, then they need 1.0-1.2 mg of RBCs or plasma.